Composite

Part:BBa_K3002225:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + GLE_Mut-PETase_SP20His + ccA_MHETase_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal NheI site found at 2665
    Illegal NheI site found at 5630
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
    Illegal NotI site found at 7043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal BglII site found at 7953
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3238
    Illegal NgoMIV site found at 3265
    Illegal NgoMIV site found at 4967
    Illegal NgoMIV site found at 6641
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002122 (GLE_Mutant PETase_SP20HA) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).

References