Composite
Part:BBa_K3002225:Design
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + GLE_Mut-PETase_SP20His + ccA_MHETase_SP20HA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5366
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6708
Illegal PstI site found at 7032
Illegal PstI site found at 7375
Illegal PstI site found at 8185 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5366
Illegal NheI site found at 2665
Illegal NheI site found at 5630
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6708
Illegal PstI site found at 7032
Illegal PstI site found at 7375
Illegal PstI site found at 8185
Illegal NotI site found at 7043 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5366
Illegal BglII site found at 7953 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5366
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6708
Illegal PstI site found at 7032
Illegal PstI site found at 7375
Illegal PstI site found at 8185 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5366
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6708
Illegal PstI site found at 7032
Illegal PstI site found at 7375
Illegal PstI site found at 8185
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3238
Illegal NgoMIV site found at 3265
Illegal NgoMIV site found at 4967
Illegal NgoMIV site found at 6641 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002122 (GLE_Mutant PETase_SP20HA) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).